Measurements were performed of vasodilation in epineurial arterioles by videomicroscopy. Group represented the normal control in which the animals were feed on a normal diet npd and had free access to water. Here, our research shows that obesity resulted in spermatogenic degeneration, imbalance of reproductive hormone, testicular oxidative stress and germ cell apoptosis, whereas evidently recovery effects. After weaning, rats were fed either a normal or highfat diet for 12 weeks. After 1 week of acclimatization, the rats were randomly divided in two groups. The same variables were then evaluated after exposing the rats to a highfat diet, after dio rats selectively developed obesity. During 3 mo on a highenergy cm diet, twothirds of the male spraguedawley rats a.
Pdf dietinduced obesity in spraguedawley rats causes. Mar 28, 2019 it remains to be demonstrated whether longterm caf diet induced obesity induces concurrent physiological and behavioural deficits. These data suggest that the clearance of cholesterol is impaired in op rats, whereas in. To achieve diet induced obesity in normal rats, harlan spraguedawley rats, purchased from charles river laboratories raleigh, nc were employed. Highfat dietinduced metabolic syndrome and oxidative stress. Obesity is a major health problem worldwide and risk factor for various cardiovascular diseases. For the study of these pathologies, a number of animal models have been developed. Marques c 1, meireles m2, norberto s2, leite j 2, freitas j 3, pestana d1, faria a4, calhau c. It was suggested that scfas may regulate gut hormones via their endogenous receptors free fatty acid receptors 2 ffar2 and 3 ffar3, but direct evidence is lacking. Obesity and overweight are associated with atherosclerosis, fatty liver, hyperlipemia, diabetes mellitus, and various types of cancer.
Weaning onto highfat diet resulted in obesity and impaired glucose tolerance in offspring exposed to prenatal stress, highfat diet, or both. Finally, central ot infusion was shown to cause body weight loss in diet induced obese mice. Nonetheless, a causal role of dietary fat has never been documented, because of inadequate animal models. In this context, a useful tool in preclinical research to study the interaction between genes and diet is represented by rats genetically prone to diet induced obesity dio and their diet resistant dr counterpart levin et al. Dietinduced adipocyte number increase in adult rats. Control c rats had free access to a standard nonpurified diet containing 51% carbohydrate, 4% fat, and 21% protein. In our study, one group of rats was fed with standard rat diet, while other group of rats fed with highfat diet for 4 weeks. Custom diet protocols can use any diet manufactured according. Dietinduced obesity alters memory consolidation in female. Highfat dietinduced metabolic syndrome and oxidative stress in. Antiobesity activity of moringa oleifera leaves against. We developed a rat model of dietinduced obesity that will be a powerful tool for. Modeling dietinduced obesity with obesityprone rats.
Animals with the most significant weight gain in the dio group were chosen in order to maximize the differences between the two groups. Furthermore, rats cotreated with ginger extract improved the histopathological and ultrastructural renal injury in duced by obesity. Pdf dietinduced obesity in rats leads to a decrease in sperm. Cromolyn sodium described as a mast cell stabilizer and chlorpheniramine maleate used as an antihistaminic and prevents allergic. A search was made for predisposing factors and sequelae of diet induced obesity dio or resistance to dio dr. In group iii, the biochemical parameters and structure of thyroid follicles were improved, and they had a nearnormal appearance. We aimed to investigate the potential efficacy of hmf on preventing obesity and hyperlipidemia in rats fed a highfat diet hfd and its underlying mechanisms. Typically mice, rats, dogs, or nonhuman primates are used in these models. Development of hypertension in a rat model of dietinduced. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Rats were singly caged with free access to water and subjected to different. In rodents, basal dopamine levels in the nac shell are lower in rats that are susceptible to diet. Postnatal diet induced obesity in rats upregulates systemic and adipose tissue glucocorticoid metabolism during development and in adulthood sandrine boulluciocca, anne dutour, viviane guillaume, vincent achard, charles oliver, michel grino.
Rat models of dietinduced obesity and high fatlow dose. To extend this line of study we investigated the development of vascular and neural complications in rats fed a high fat diet. Mechanisms underlying the resistance to dietinduced. The global prevalence of overweight and obesity has reached epidemic proportions. Since rats and mice show a similar relationship, they are considered an appropriate model. Highfructose and highfat diet induced disorders in rats. Effect of highfat dietinduced obesity on thyroid gland. In this study, we examined the effects of lactose on longterm highfat diet induced obesity in rats. High fatstz rodent models fat fedstz diabetic rodents, developed by combination of diet induced insulin resistance and relatively lowdose streptozotocin stz provide a novel animal model for diet induced type 2 diabetes. Frontiers highfat dietinduced obesity model does not. Ontogeny of dietinduced obesity in selectivelybred rats.
Gf mice are protected against diet induced obesity. Rats genetically prone to diet induced obesity dio and their counterpart diet resistant dr were fed a chow diet, and their response to damphetamine on intracranial selfstimulation and food intake were assessed. We sought to determine the net effects of dietary supplementation of leucine on wholebody macronutrient and energy metabolism in mice with free access to a highfat diet hfd and to assess how cardiovascular risks associated with dietinduced obesity may be modified by increased dietary leucine intake. Control males are housed in the same location, also at reduced density, and are fed nih31m diet control males can also be generated using a low fat purified diet upon request. To assess the effects of diet induced obesity dio, on the biomechanical and biochemical properties of the femur in mature male rats. May 28, 20 crosstalk between akkermansia muciniphila and intestinal epithelium controls dietinduced obesity. Pdf highfat dietinduced obesity in animal models researchgate. Obesity induced by highfat diet in female rats structurally and functionally changed the thyroid gland in a way that may explain hypothyroidism associated with obesity. Overfeeding induced earlyonset obesity and accelerated the maturation of the hypothalamopituitaryadrenal hpa axis together. Shortchain fatty acids protect against highfat dietinduced. Mar 20, 2019 obesity induced by a highfat diet had a substantial elevation in adiposity parameters, fe, lv, and lvwtibia length when compared to c rats.
Thus, inflammation, as well as other comorbidities, may provide a. Jul 10, 2012 society for the study of ingestive behavior. Highfat dietinduced metabolic syndrome and oxidative. In addition, hfhf and hfd induced obesity associated with hyperleptinaeemia and endothelial dysfunction. Excess caloric intake increases the amount of adipose tissue and contributes to metabolic disorders in disrupted metabolic homeostasis. Since rats and mice show a similar relationship, they are considered an appropriate model for studying dietary obesity. Cqa on lipid metabolism in the liver of spraguedawley rats fed a high. Effects of ginseng dietary supplementation on a highfat. Rats fed on the normal diet and hed continued to show increased body weight fig. Prenatal stress or highfat diet increases susceptibility. Beneficial effects of cocoa, coffee, green tea, and. The impact of the mitochondriatargeted antioxidant mitoq was evaluated in the cardiac alterations associated with obesity. Effects of dietary lactose on longterm highfatdiet. Thus, rats consuming the hf diet gained increased body fat relative to rats eating either of two quite different lf diets.
Effects of different diets used in dietinduced obesity models on. When compared over 10 wk of free feeding, hf rats weighed 10% more p. Mitochondrial oxidative stress in the cardiac alterations. Keywords spraguedawley rat, dietinduced obesity, toxicology parameters, bone toxicity. Induction of obesity by feeding high fat diet experimental obesity was induced by feeding of high fat diet consisting of the following materials corn starch, casein, lard, cholesterol, sodium cholate, soybean oil, coconut oil, wheat bran, mineral mix, vitamin mix were added to 1kg of rat chow diet to rats. Dietinduced obesity in rats leads to a decrease in sperm motility article pdf available in reproductive biology and endocrinology 91. Mechanisms of the antiobesity effects of oxytocin in diet. Group 1 represented the normal control in which the animals were feed on a normal diet npd and had free access to water.
The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Highfat diet induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation skip to main content thank you for visiting. The first group had free access to a standard diet normal diet nd from safe augy, france, with the following macronutrient composition. The highfat diet resulted in 20% increased body weight.
Highfat diet hfd induced obesity in rats is considered to be a reliable tool for the evaluation of antiobesity activity. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. In this study, we examined the effects of lactose on long. Fdio rats represent a novel and potentially useful model for the study of diet induced obesity and insulin resistance. Using chowfed lean rats and two groups of highfat diet hfdfed rats with. To determine whether gf mice are protected against diet induced obesity, adult c57bl6j males, maintained since weaning on an autoclaved lowfat chow diet 5% lipids. Pdf the effect of highfat dietinduced obesity on cardiovascular. In our study, one group of rats was fed with standard rat diet, while other group of rats fed with. The objective was to determine the effect of diet induced obesity dio on microvascular and neural function. Peripherally infused lean and hfdinduced obese rats received.
The effect of dietinduced obesity on toxicological parameters in. We applied a comparative functional genomics approach to evaluate whether diet. Inquire about customized diet induced obese dio models using any of taconics commercial animal models. Effects of food pattern change and physical exercise on. Pdf ginger extract ameliorates renal damage in high fat. Thus, the objective of this study was to evaluate the effect of a chronic 20week caf feeding period on metabolic and cognitive functioning in a spraguedawley rats. Finally, we comment on the reversibility of highfat diet induced obesity. The two rat models used in these studies were the diet induced obese and type 2.
Highfat diet hfd induced obesity in rats was an effective model to evaluate anti obesity activities of drugs. Postnatal diet induced obesity in rats upregulates. Dietinduced obese dio rat model a unique dio rat model. This study aimed to investigate the anti obesity effects of ginseng and the alternation of gut microbiota composition in highfat diet hfd induced obesity. Highfructose and highfat dietinduced disorders in rats. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. However, the mechanisms underlying such beneficial effects of. Epidemiological studies have shown a positive relationship between dietary fat intake and obesity. The physiologic characteristics of the diet induced obesity rat model are insulin resistance, impaired glucose tolerance, dyslipidemia and increased fat deposition. In the aorta, nox4 expression was increased by threefold in obese rats. Highfatdietinduced obesity causes an inflammatory and tumor. The study comprises 5 groups with 10 animals in each group.
Dietinduced models for obesity and type 2 diabetes. They were housed in individual metabolic cages nalgene, rochester, ny with a constant temperature and 12 h of light altering with 12 h of darkness. As the rats mature, growth rates slow, the gain in body weight and fatfree. Mekalanos, harvard medical school, boston, ma, and approved november 22, 2006.
The average food and water intake of each rat are presented in table 1. The first description of a highfat diet to induce obesity by a nutritional intervention. From genetics to physiology article pdf available in obesity 202. The total calories contained in 100 g of each diet were 349 and 365 kcal in the cg and hs, respectively. Effect of consumption of a highfat hf diet on body weight, adiposity, and food intake in lowfat lf and hffed animals after 8 or 12 wk on respective diets a. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Butyrate and propionate protect against dietinduced obesity.
Systemic upregulation of nadph oxidase in dietinduced. Greater dietinduced obesity in rats consuming sugar solution. Diet induced obesity in sprague dawley rats causes. Controlled highfat diet induces an obese syndrome in rats.
Oxidative stress in a rat model of obesityinduced hypertension. In the present study our rats also developed a concurrent range of nerve disorders, including sncv slowing, thermal hypoalgesia. Modeling dietinduced obesity with obesityprone rats ncbi. Diet induced obesity in rats is also reported to impact the brain in rats 24, as well as alter serum adipokine levels 25,26. Results are consistent with other reports of diet induced obesity but only after 8 weeks of a hypercholesterolemic diet. After 4week induced obesity, the body weight was higher for hed than control rats p obesity and its associated diabetes in humans. Mechanisms of the antiobesity effects of oxytocin in dietinduced. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. Despite the differences between the metabolism and gpr affinities of the three scfas, they ameliorate highfat diet hfdinduced obesity and insulin resistance to a similar extent when given as a dietary supplement 1517. A nonobese, dietinduced animal model of nonalcoholic. Antiobesity activity of liraglutide on high fat diet. However, similar plasma triglyceride, plasma free fatty acid and renal. Table 1 a different types of diet used and dietinduced body and metabolic alterations dietinduced obesity.
Activation of the systemic and adipose reninangiotensin system in rats with dietinduced obesity and hypertension. These differences were even more marked relative to rats fed the np diet, the control traditionally used in studies of rodent dietinduced obesity. Increasing dietary leucine intake reduces dietinduced. Cardiovascular function in a rat model of dietinduced obesity. Propensity to highfat diet induced obesity in rats is associated with changes in the gut microbiota and gut in. Expression of new loci associated with obesity in diet induced obese rats. Pdf energy expenditure in rats with dietinduced obesity.
Modified highsucrose dietinduced abdominally obese and. Rats were fed a standard or high fat diet for up to 32 weeks. Diet induced obesity in spraguedawley rats causes microvascular and neural dysfunction. At the age of 3 weeks, male long evans rats were assigned to the high. A controlled highfat diet induces an obese syndrome in rats. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. The increase in free fatty acids flux to the liver from an expanded.
The consumption of a highfat diet hfd is considered a risk factor for obesity development. In contrast, the fc was lower in ob than in c rats. Obese rats model induced by highfat diets were established and gavage treated with 0, 50,150 or 450 mg of sifkg body weightday for 4 weeks. Lf, p nov 24, 2016 in contrast, selection from outbred strains presupposes greater variability in both genetic and epigenetic differences, which likely better reflects the susceptibility to obesity in humans. The effect of highfat dietinduced obesity on cardiovascular. Together, the data suggest that maternal prenatal stress or highfat diet alters susceptibility of offspring to diet induced obesity and its metabolic consequences. Molecules free fulltext soy isoflavones improve the. Therefore, our aim has been to characterize the interactions between cardiolipotoxicity and mitochondrial oxidative stress in the cardiac alterations in diet induced obese rats. Fat fedstz diabetic rodents, developed by combination of diet induced insulin resistance and relatively lowdose streptozotocin stz provide a novel animal model for diet induced type 2 diabetes.
Estradiol replacement improves highfat dietinduced. Pdf obesity is rapidly becoming a worldwide epidemic that affects children and adults. Research article antiobesity and hypolipidemic activity of. Effects of food pattern change and physical exercise on cafeteria dietinduced obesity in female rats volume 108 issue 8 jeferson f. We examined the effects of scfa administration in mice, and show that butyrate, propionate, and acetate all protected against dietinduced obesity and insulin resistance. Diet induced adipocyte number increase in adult rats. Dawley strain female rats 6 weeks old were divided into four groups. However, little is known about the contribution of 3,5,6,7,8,3. It was an animal experimental study and was carried out in the of pharmacology and. However, hfd alone induced only transient insulin resistance, glucose intolerance, and hypertriglyceridaemia, without. The hs diet induced increased visceral adipose tissue without increased body weight, reduced glucose disposal rates, and increased hepatic glucose output during the hyperinsulinemiceuglycemic clamp, increased plasma glucose during the. Combined effect of cromolyn sodium and chlorpheniramine. This suggests that there is a common, gprindependent molecular mechanism for the beneficial effects of acetate.
Cafeteriadiet induced obesity results in impaired cognitive. The major factors that contribute to dietary obesity hyperphagia, energy density and. Pdf dietinduced obesity in rats leads to a decrease in. Initiation and perpetuation of obesity and obesity. Energy expenditure in rats with dietinduced obesity article pdf available in american journal of clinical nutrition 442. Raybould3 3department of anatomy, physiology, and cell biology, university of california davis school of veterinary medicine, davis. Additionally, plasma ot levels were reported to be decreased in diet induced obese mice and increased in synaptotagmin4 deficient mice that are protected against diet induced obesity. Pdf expression of new loci associated with obesity in. Antiobesity and hypolipidemic activity of moringa oleifera. Values of a corresponding parameter are significantly different at all time points p diet induced leptin resistance also interferes with memory consolidation in rats, such that marked deficits are observed in object recognition tests in diet induced obese rats compared to wild. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Rodent model for the study of obesityrelated disorders 387rev assoc med bras 2012. When fed ad libitum with a highfat diet hfd, obesity, hyperinsulinemia, hyperglycemia and hypertension establish in these mice. Young male spraguedawley rats were fed chow or a modified highsucrose hs diet for 20 weeks.
Dec 27, 2019 highfat diet induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation skip to main content thank you for visiting. Adrenals were dissected free of fat, weighed to the nearest milligram, and homogenized in a solution of 0. Greater dietinduced obesity in rats consuming sugar solution during the inactive period. Many rat strains show variability in weight gain when fed a highenergy diet 21. A unique dio rat model to evaluate your drugs on obesity and cardiometabolic complications key benefits demonstrate the efficacy of your drugs in the physiogenex free choice diet induced obese dio rat. Mar 18, 2019 diet induced obese dio rats have altered stress hpa axis activity compared to diet resistant dr rats when chronically exposed to a highfat hf diet. Dietinduced obese dio rat model a unique dio rat model to. The study concluded that the ginger extract used could suppress and decrease the renal damage induced by highfat diet as it possesses potential medicinal values. Male wistar rats were fed either a high fat diet hfd, 35% fat or a standard diet ct, 3. Responsiveness of hypothalamopituitaryadrenal axis to. Rodent model for the study of obesity related disorders 387rev assoc med bras 2012. It is intended to mimic the most common cause of obesity in humans. After rats had ad libitum access to hfd for 6 wk to develop hfd induced obesity and impaired glucose tolerance, we delivered the vector aavshnpy into the dmh of hfd induced obese rats for speci.
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